Journal: Current Protocols
Article Title: Analysis of Reactive Oxygen Species–Induced Cellular Damage in Cervical Cancer
doi: 10.1002/cpz1.70328
Figure Lengend Snippet: Quantitative analysis of total superoxide dismutase (T‐SOD) activity (U/mg protein) in the presence of 10 µM 25(OH)D 3 (yellow) and 250 µM calcipotriol (red).
Article Snippet: Total SOD Activity Assay kit (hydroxylamine method, Elabscience, cat. no. E‐BC‐K019‐M) 1× PBS (see recipe) 96‐well plates, clear, flat‐bottom, polystyrene (Lasec, cat. no. P1PLA044C‐000096ST) 1.5‐ml microcentrifuge tubes (Separations, cat. no. VWRA525‐1528) Humidified 37°C, 5% CO 2 incubator (water jacket type, Astec Bio, model WCl‐165) ELx800 Universal Microplate Reader (Bio‐Tek Instruments)
Techniques: Activity Assay